Photodynamic therapy using a novel irradiation source, LED lamp, is similarly effective to photodynamic therapy using diode laser or metal-halide lamp on DMBA- and TPA-induced mouse skin papillomas

J Dermatol. 2014 Aug;41(8):729-31. doi: 10.1111/1346-8138.12572.


Photodynamic therapy (PDT) is useful for superficial skin tumors such as actinic keratosis and Bowen disease. Although PDT is non-surgical and easily-performed treatment modality, irradiation apparatus is large and expensive. Using 7, 12-dimethylbenz[a]anthracene (DMBA) and 12-ο-tetradecanoylphorbol-13-acetate (TPA)-induced mouse skin papilloma model, we compared the efficacy of TONS501- and ALA-PDT with a LED lamp, a diode laser lamp or a metal-halide lamp on the skin tumor regression. TONS501-PDT using 660 nm LED lamp showed anti-tumor effect at 1 day following the irradiation and the maximal anti-tumor effect was observed at 3 days following the irradiation. There was no significant difference in the anti-tumor effects among TONS501-PDT using LED, TONS501-PDT using diode laser, and 5-aminolevulinic acid hydrochloride (ALA)-PDT using metal-halide lamp. Potent anti-tumor effect on DMBA- and TPA-induced mouse skin papilloma was observed by TONS501-PDT using 660 nm LED, which might be more useful for clinical applications.

Keywords: 5-aminolevulinic acid; LED lamp; TONS501; photodynamic therapy; skin tumor.

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene / toxicity
  • Aminolevulinic Acid / therapeutic use
  • Animals
  • Female
  • Lasers, Semiconductor / therapeutic use
  • Mice
  • Mice, Hairless
  • Papilloma / chemically induced
  • Papilloma / drug therapy*
  • Photochemotherapy / instrumentation*
  • Photochemotherapy / methods
  • Photosensitizing Agents / therapeutic use
  • Porphyrins / therapeutic use
  • Skin Neoplasms / chemically induced
  • Skin Neoplasms / drug therapy*
  • Tetradecanoylphorbol Acetate / toxicity


  • 13,17-bis(1-carboxypropionyl)carbamoylethyl-3-ethenyl-2-hydroxy-3-hydroxyiminoethlidene-2,7,12,18-tetramethylporphyrin
  • Photosensitizing Agents
  • Porphyrins
  • 9,10-Dimethyl-1,2-benzanthracene
  • Aminolevulinic Acid
  • Tetradecanoylphorbol Acetate