Abstract
Tea polyphenols are functional substances present in tea. Kuding tea as a traditional drink also contains these compounds. After 25, 50 and 100 μg/mL of Kuding tea polyphenol treatment for 48 h, cell proliferation of human buccal squamous cell carcinoma cell line BcaCD885 was inhibited, and the 100 μg/mL of Kuding tea polyphenol showed the highest inhibitory rate at 72.3%. Compared to the lower concentration, the 100 μg/mL of Kuding tea polyphenols significantly (p < 0.05) induced apoptosis as determined by flow cytometry analysis, the content of sub-G1 cancer cells was 32.7%. By RT-PCR and western blot assays, Kuding tea polyphenol significantly induced apoptosis in BcaCD885 cancer cells (p < 0.05) by upregulating caspase-3, caspase-8, caspase-9, Fas/FasL, Bax, p53, p21, E2F1, p73 and downregulating Bcl-2, Bcl-xL, HIAP-1, and HIAP-2 mRNA and protein expressions. Kuding tea polyphenols thus present apoptosis inducing effects in vitro.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antineoplastic Agents / pharmacology
-
Apoptosis / drug effects*
-
Apoptosis Regulatory Proteins / genetics
-
Apoptosis Regulatory Proteins / metabolism
-
Carcinoma, Squamous Cell / metabolism
-
Catechin / pharmacology
-
Cell Line, Tumor
-
Cell Proliferation
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism
-
Down-Regulation
-
E2F1 Transcription Factor / genetics
-
E2F1 Transcription Factor / metabolism
-
Fas Ligand Protein / genetics
-
Fas Ligand Protein / metabolism
-
Humans
-
Inhibitor of Apoptosis Proteins / genetics
-
Inhibitor of Apoptosis Proteins / metabolism
-
Nuclear Proteins / genetics
-
Nuclear Proteins / metabolism
-
Plant Extracts / pharmacology
-
Polyphenols / pharmacology*
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Tea / chemistry*
-
Tumor Protein p73
-
Tumor Suppressor Protein p53 / genetics
-
Tumor Suppressor Protein p53 / metabolism
-
Tumor Suppressor Proteins / genetics
-
Tumor Suppressor Proteins / metabolism
-
Up-Regulation
Substances
-
Antineoplastic Agents
-
Apoptosis Regulatory Proteins
-
DNA-Binding Proteins
-
E2F1 Transcription Factor
-
E2F1 protein, human
-
FASLG protein, human
-
Fas Ligand Protein
-
Inhibitor of Apoptosis Proteins
-
Nuclear Proteins
-
Plant Extracts
-
Polyphenols
-
RNA, Messenger
-
TP53 protein, human
-
TP73 protein, human
-
Tea
-
Tumor Protein p73
-
Tumor Suppressor Protein p53
-
Tumor Suppressor Proteins
-
Catechin