Application of a computational neural network to optimize the fluorescence signal from a receptor-ligand interaction on a microfluidic chip

Electrophoresis. 2015 Feb;36(3):393-7. doi: 10.1002/elps.201400288. Epub 2014 Oct 2.

Abstract

We describe the use of a computational neural network platform to optimize the fluorescence upon binding 5-carboxyfluorescein-d-Ala-d-Ala-d-Ala (5-FAM(DA)3 ) (1) to the antibiotic teicoplanin covalently attached to a glass slide. A three-level response surface experimental design was used as the first stage of investigation. Subsequently, three defined experimental parameters were examined by the neural network approach: (i) the concentration of teicoplanin used to derivatize a glass platform on the microfluidic device, (ii) the time required for the immobilization of teicoplanin on the platform, and (iii) the length of time 1 is allowed to equilibrate with teicoplanin in the microfluidic channel. Optimal neural structure provided a best fit model, both for the training set (r(2) = 0.961) and test set (r(2) = 0.934) data. Model simulated results were experimentally validated with excellent agreement (% difference) between experimental and predicted fluorescence shown, thus demonstrating efficiency of the neural network approach.

Keywords: Computational neural networks; Microfluidics; Optimization; Receptor-ligand binding.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies, Immobilized / chemistry*
  • Antibodies, Immobilized / metabolism
  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Microfluidic Analytical Techniques / instrumentation*
  • Neural Networks, Computer
  • Oligopeptides / chemistry
  • Oligopeptides / metabolism
  • Protein Binding
  • Spectrometry, Fluorescence / instrumentation*
  • Teicoplanin / chemistry
  • Teicoplanin / metabolism

Substances

  • Antibodies, Immobilized
  • Fluorescent Dyes
  • Oligopeptides
  • carboxyfluorescein-D-Ala-D-Ala-D-Ala
  • Teicoplanin