Distinguishing closely related amyloid precursors using an RNA aptamer

J Biol Chem. 2014 Sep 26;289(39):26859-26871. doi: 10.1074/jbc.M114.595066. Epub 2014 Aug 6.


Although amyloid fibrils assembled in vitro commonly involve a single protein, fibrils formed in vivo can contain multiple protein sequences. The amyloidogenic protein human β2-microglobulin (hβ2m) can co-polymerize with its N-terminally truncated variant (ΔN6) in vitro to form hetero-polymeric fibrils that differ from their homo-polymeric counterparts. Discrimination between the different assembly precursors, for example by binding of a biomolecule to one species in a mixture of conformers, offers an opportunity to alter the course of co-assembly and the properties of the fibrils formed. Here, using hβ2m and its amyloidogenic counterpart, ΔΝ6, we describe selection of a 2'F-modified RNA aptamer able to distinguish between these very similar proteins. SELEX with a N30 RNA pool yielded an aptamer (B6) that binds hβ2m with an EC50 of ∼200 nM. NMR spectroscopy was used to assign the (1)H-(15)N HSQC spectrum of the B6-hβ2m complex, revealing that the aptamer binds to the face of hβ2m containing the A, B, E, and D β-strands. In contrast, binding of B6 to ΔN6 is weak and less specific. Kinetic analysis of the effect of B6 on co-polymerization of hβ2m and ΔN6 revealed that the aptamer alters the kinetics of co-polymerization of the two proteins. The results reveal the potential of RNA aptamers as tools for elucidating the mechanisms of co-assembly in amyloid formation and as reagents able to discriminate between very similar protein conformers with different amyloid propensity.

Keywords: Amyloid; Amyloid Fibril; Amyloid Precursor; Aptamer; Co-polymerization; Protein Aggregation; Protein Folding; RNA Aptamer; Structural Biology; β2-Microglobulin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid / chemistry*
  • Aptamers, Nucleotide / chemistry*
  • Humans
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Multimerization*
  • beta 2-Microglobulin / chemistry*


  • Amyloid
  • Aptamers, Nucleotide
  • beta 2-Microglobulin