Antidiabetic effect of Lactobacillus casei CCFM0412 on mice with type 2 diabetes induced by a high-fat diet and streptozotocin

Nutrition. 2014 Sep;30(9):1061-8. doi: 10.1016/j.nut.2014.03.022. Epub 2014 Mar 31.


Objective: The aim of this study was to evaluate the antidiabetic effects of Lactobacillus casei CCFM0412 on mice with type 2 diabetes induced by a high-fat diet and streptozotocin.

Methods: Thirty-two male C57 BL/6 J mice were assigned to four groups in this study. Type 2 diabetes was induced by feeding of a high-fat diet and injection of streptozotocin. L. casei CCFM0412 was administered to mice at a dose of 10(9) cfu/d per mouse for 12 wk. Body weight, fasting and postprandial 2-h blood glucose, oral glucose tolerance, glycosylated hemoglobin, insulin, and glycogen in liver were measured. Endotoxin, tumor necrosis factor-α, and interleukin-10 levels were determined. Lipid metabolic parameters including triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were also measured. The activities of glutathione peroxides, reactive oxygen species, and superoxide dismutase, and the levels of glutathione and malondialdehyde in the liver also were determined. Pancreas injury was evaluated by histologic analysis.

Results: At 13 wk, L. casei CCFM0412 significantly decreased fasting and postprandial 2-h blood glucose, glycosylated hemoglobin, endotoxin, tumor necrosis factor-α, triglycerides, total cholesterol, low-density lipoprotein cholesterol, reactive oxygen species, and malondialdehyde levels compared with the control group (P < 0.05). The values for insulin, interleukin-10, high-density lipoprotein cholesterol, glutathione peroxides, superoxide dismutase, glutathione, and glycogen were significantly increased at 13 wk (P < 0.05). Islets of Langerhans in the L. casei CCFM0412 group were substantially protected from destruction compared with those in the control group.

Conclusion: L. casei CCFM0412 significantly improved glucose intolerance, dyslipidemia, immune-regulatory properties, and oxidative stress in mice with type 2 diabetes induced by a high-fat diet and streptozotocin. The results provide a sound rationale for future clinical trials of oral administration of L. casei CCFM0412 for the primary prevention of type 2 diabetes.

Keywords: Antidiabetic effect; Blood glucose; Lactobacillus casei CCFM0412; Pioglitazone; Type 2 diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Blood Glucose / metabolism*
  • Diabetes Mellitus, Experimental / blood
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / drug therapy*
  • Diabetes Mellitus, Type 2 / etiology
  • Diet, High-Fat*
  • Dyslipidemias / drug therapy
  • Dyslipidemias / etiology
  • Endotoxins / blood
  • Glucose Intolerance / blood
  • Glucose Intolerance / drug therapy
  • Glucose Intolerance / etiology
  • Glycated Hemoglobin / metabolism
  • Hypoglycemic Agents / therapeutic use*
  • Insulin / blood
  • Islets of Langerhans / drug effects
  • Lacticaseibacillus casei*
  • Lipids / blood
  • Male
  • Malondialdehyde / blood
  • Mice, Inbred C57BL
  • Oxidative Stress / drug effects
  • Streptozocin
  • Tumor Necrosis Factor-alpha / blood


  • Antioxidants
  • Blood Glucose
  • Endotoxins
  • Glycated Hemoglobin A
  • Hypoglycemic Agents
  • Insulin
  • Lipids
  • Tumor Necrosis Factor-alpha
  • Malondialdehyde
  • Streptozocin