Rabbiteye blueberry prevents osteoporosis in ovariectomized rats

J Orthop Surg Res. 2014 Aug 8;9:56. doi: 10.1186/s13018-014-0056-9.


Objective: It has been forecasted that the rabbiteye blueberry could inhibit osteoporosis. However, the inhibition and prevention of osteoporosis via rabbiteye blueberry are still elusive. This study was aim to evaluate the anti-osteoporosis effects of rabbiteye blueberry in ovariectomized rats.

Methods: Thirty rats were randomly divided into three groups of ten rats each as follows: sham-operated group (SG), ovariectomized model control group (OMG), and ovariectomized rabbiteye blueberry treatment group (OBG). The blood mineral levels, the alkaline phosphatase (ALP) activity, and osteoprotegerin (OPG) level were determined. The expression analyses of type I collagen, integrin-β1, and focal adhesion kinase (FAK) were performed. Besides, the bone mineral density (BMD) and bone histomorphometry (BH) were measured.

Results: The ALP activity in SG and OBG was significantly lower than that in OMG. For the OPG level, the significant increase of OPG level in OBG was indicated compared with the other groups. The mRNA expression levels of type I collagen, integrin-β1, and FAK in OMG were significantly lower than those in other groups. The BMD in OMG were all significantly lower than those in SG and OBG. For BH, blueberry significantly improved the trabecular bone volume fraction, trabecular thickness, mean trabecular bone number, and bone formation rate, and decreased the trabecular separation, the percent of bone resorption perimeter, and mean osteoclast number in OBG compared with OMG.

Conclusions: The rabbiteye blueberries had an effective inhibition in bone resorption, bone loss, and reduction of bone strength of ovariectomized rats and could improve the BMD, osteogenic activity, and trabecular bone structure.

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Blueberry Plants*
  • Bone Density
  • Disease Models, Animal
  • Female
  • Humans
  • Osteoporosis, Postmenopausal / prevention & control*
  • Osteoprotegerin / metabolism
  • Ovariectomy
  • Rats, Sprague-Dawley


  • Osteoprotegerin
  • Alkaline Phosphatase