Enhancement of L-cysteine production by disruption of yciW in Escherichia coli

J Biosci Bioeng. 2015 Feb;119(2):176-9. doi: 10.1016/j.jbiosc.2014.07.006. Epub 2014 Aug 4.


Using in silico analysis, the yciW gene of Escherichia coli was identified as a novel L-cysteine regulon that may be regulated by the transcriptional activator CysB for sulfur metabolic genes. We found that overexpression of yciW conferred tolerance to L-cysteine, but disruption of yciW increased L-cysteine production in E. coli.

Keywords: CysB-binding motif; Escherichia coli; In silico analysis; l-Cysteine fermentation; yciW gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Computer Simulation
  • Cysteine / metabolism*
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Fermentation
  • Regulon / genetics
  • Sulfur / metabolism
  • Trans-Activators / metabolism


  • Bacterial Proteins
  • CysB protein, Bacteria
  • Trans-Activators
  • Sulfur
  • Cysteine