Using in silico analysis, the yciW gene of Escherichia coli was identified as a novel L-cysteine regulon that may be regulated by the transcriptional activator CysB for sulfur metabolic genes. We found that overexpression of yciW conferred tolerance to L-cysteine, but disruption of yciW increased L-cysteine production in E. coli.
Keywords: CysB-binding motif; Escherichia coli; In silico analysis; l-Cysteine fermentation; yciW gene.
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