Pedobacter xixiisoli sp. nov., isolated from bank soil

Int J Syst Evol Microbiol. 2014 Nov;64(Pt 11):3683-3689. doi: 10.1099/ijs.0.065946-0. Epub 2014 Aug 8.

Abstract

A Gram-stain-negative, rod-shaped, yellow, non-motile, aerobic bacterium (strain S27(T)) was isolated from bank soil of the Xixi wetland in Zhejiang province, PR China. Phylogenetic analysis, based on its 16S rRNA gene sequence, revealed that strain S27(T) could represent a novel species of the genus Pedobacter showing highest similarity to Pedobacter koreensis WPCB189(T) (95.45%), followed by 'Pedobacter zeaxanthinifaciens' TDMA-5 (95.22%). The temperature, pH and NaCl concentration ranges for growth were 6-37 °C (optimum 28 °C), pH 5.0-9.0 (optimum pH 7.5) and 0-3% (w/v) [optimum 0.5% (w/v)], respectively. The DNA G+C content was 36.1 mol%, MK-7 was the only respiratory quinone, and iso-C(15:0), iso-C(17:0) 3-OH and summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH) were the major fatty acids. These data all support the affiliation of strain S27(T) to the genus Pedobacter. The polar lipids of strain S27(T) comprised phosphatidylethanolamine, one unidentified aminophospholipid, four unidentified aminolipids and three unidentified lipids. However, strain S27(T) could be distinguished from other members of the genus Pedobacter due to its physiological and biochemical characteristics. Therefore, strain S27(T) represents a novel species of the genus Pedobacter, for which the name Pedobacter xixiisoli sp. nov. is proposed; the type strain is S27(T) ( =CGMCC 1.12803(T) =NBRC 110388(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • China
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Molecular Sequence Data
  • Pedobacter / classification*
  • Pedobacter / genetics
  • Pedobacter / isolation & purification
  • Phylogeny*
  • Pigmentation
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry
  • Wetlands*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • vitamin MK 7

Associated data

  • GENBANK/KJ150595