Biochemical analysis of transcriptional activation by Jun: differential activity of c- and v-Jun

Cell. 1989 Nov 17;59(4):709-17. doi: 10.1016/0092-8674(89)90017-2.

Abstract

The human proto-oncogene product, c-Jun, is a member of the AP-1 family of transcription factors, which mediate the regulation of gene expression in response to extracellular signaling. Comparison of c-Jun and v-Jun by in vitro transcription assays revealed that v-Jun has significantly greater transcriptional activity than c-Jun. Analysis of Jun mutants expressed in bacteria indicates that this difference in transcriptional activity is due to the presence of a regulatory domain located at the N-terminal region of c-Jun. Other Jun mutants identify an activation domain rich in acidic and proline residues toward the C-terminal end of the molecule, in a region near the DNA binding domain. These findings suggest that during retroviral transduction, a constitutively active Jun protein has been generated by deleting a negatively acting domain. This putative repressor domain may also play a role in the signal-dependent induction of c-Jun activity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cell Transformation, Viral
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / genetics
  • Gene Expression Regulation*
  • HeLa Cells / metabolism
  • Humans
  • Molecular Sequence Data
  • Mutation
  • Oncogene Protein p65(gag-jun)
  • Plasmids
  • Protein Processing, Post-Translational
  • Protein-Tyrosine Kinases / metabolism
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-jun
  • Proto-Oncogenes*
  • Retroviridae Proteins, Oncogenic / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic*

Substances

  • DNA-Binding Proteins
  • Oncogene Protein p65(gag-jun)
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-jun
  • Retroviridae Proteins, Oncogenic
  • Transcription Factors
  • Protein-Tyrosine Kinases