Multiple GF-1 binding sites flank the erythroid specific transcription unit of the human carbonic anhydrase I gene

FEBS Lett. 1989 Nov 6;257(2):451-6. doi: 10.1016/0014-5793(89)81594-7.

Abstract

Six potential GF-1 sites which bind an erythroid factor are present in the 5' and 3' regions flanking the erythroid-specific transcription unit of the human carbonic anhydrase I (HCAI) gene. When two of these sites are placed upstream of a minimal eukaryotic promoter they confer up-regulated expression in erythroid over non-erythroid cells. The presence of the erythroid factor in TPA-treated HEL cells in which the level of HCAI transcript has greatly decreased and in non-HCAI-expressing K562 cells suggests that in these cases the presence of the factor is not sufficient for HCAI expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Carbonic Anhydrases / genetics*
  • Cell Line
  • Erythrocytes / physiology*
  • Gene Expression Regulation
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping
  • Transcription Factors / physiology*
  • Transcription, Genetic

Substances

  • Transcription Factors
  • Carbonic Anhydrases