Brief exposure to cigarette smoke impairs airway epithelial cell innate anti-viral defence

Toxicol In Vitro. 2014 Dec;28(8):1430-5. doi: 10.1016/j.tiv.2014.07.012. Epub 2014 Aug 8.

Abstract

Background: Human rhinovirus (hRV) infections commonly cause acute upper respiratory infections and asthma exacerbations. Environmental cigarette smoke exposure is associated with a significant increase in the risk for these infections in children.

Objective: To determine the impact of short-term exposure to cigarette smoke on innate immune responses of airway epithelial cells infected with hRV.

Methods: A human bronchial epithelial cell line (HBEC-3KT) was exposed to cigarette smoke extract (CSE) for 30 min and subsequently infected with hRV serotype 1B. Viral-induced cytokine release was measured with AlphaLISA and viral replication quantified by shed viral titer and intracellular viral copy number 24h post-infection.

Results: CSE induced a concentration-dependent decrease in CXCL10 (p<0.001) and IFN-β (p<0.001), with a 79% reduction at the highest dose with an associated 3-fold increase in shed virus. These effects were maintained when infection was delayed up to 24h post CSE exposure. Exogenous IFN-β treatment at t=0 after infection blunts the effects of CSE on viral replication (p<0.05).

Conclusion: A single exposure of 30 min to cigarette smoke has a lasting impact on epithelial innate defence providing a plausible mechanism for the increase in respiratory infections seen in children exposed to second-hand tobacco smoke.

Keywords: Cigarette smoke; Epithelial cells; Innate immunity; Interferon-β; Rhinovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bronchi / immunology*
  • Cells, Cultured
  • Chemokine CXCL10 / genetics
  • Epithelial Cells / immunology
  • Humans
  • Immunity, Innate*
  • Interferon-beta / metabolism
  • Interferon-beta / pharmacology
  • Interleukin-8 / metabolism
  • Rhinovirus / immunology*
  • Smoke / adverse effects*
  • Tobacco / adverse effects*

Substances

  • CXCL10 protein, human
  • Chemokine CXCL10
  • Interleukin-8
  • Smoke
  • Interferon-beta