Radiometric studies on the use of selective inhibitors in the identification of Mycobacterium spp

J Med Microbiol. 1989 Nov;30(3):175-81. doi: 10.1099/00222615-30-3-175.


Radiometric selective inhibition tests were developed and evaluated for the rapid differentiation of Mycobacterium spp. Both a p-nitrobenzoic acid (PNB) test and a commercially-prepared p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (NAP) test successfully differentiated M. tuberculosis and M. bovis from "atypical" mycobacteria or mycobacteria other than tubercle bacilli (MOTT). Thiophene-2-carboxylic acid hydrazide (TCH) readily distinguished human M. tuberculosis strains from M. bovis, irrespective of resistance to isoniazid. Both PNB and TCH tests were utilised in a routine radiometric susceptibility testing scheme over a period of 1 year in which 110 isolates of M. tuberculosis, 10 of M. bovis and one isolate of BCG were correctly differentiated from 10 isolates of MOTT. The rapidity, sensitivity and specificity of these radiometric tests can play a useful role in mycobacterial identification.

Publication types

  • Comparative Study

MeSH terms

  • Drug Resistance, Microbial
  • Humans
  • Hydroxypropiophenone / pharmacology
  • Isoniazid / pharmacology
  • Microbial Sensitivity Tests
  • Mycobacterium / classification*
  • Mycobacterium / drug effects
  • Mycobacterium avium Complex / classification
  • Mycobacterium avium Complex / drug effects
  • Mycobacterium bovis / classification
  • Mycobacterium bovis / drug effects
  • Mycobacterium tuberculosis / classification
  • Mycobacterium tuberculosis / drug effects
  • Nitrobenzoates / pharmacology*
  • Nontuberculous Mycobacteria / classification*
  • Nontuberculous Mycobacteria / drug effects
  • Radiometry
  • Species Specificity
  • Thiophenes / pharmacology*


  • Nitrobenzoates
  • Thiophenes
  • Hydroxypropiophenone
  • thiophene-2-carboxylic acid hydrazide
  • 4-nitro-alpha-acetylamino-beta-hydroxypropiophenone
  • 4-nitrobenzoic acid
  • Isoniazid