Enhancing the interaction between annexin-1 and formyl peptide receptors regulates microglial activation to protect neurons from ischemia-like injury

Zhen Zhao Luo; Yan Gao; Ning Sun; Yin Zhao; Jing Wang; Bo Tian; Jing Shi

doi:10.1016/j.jneuroim.2014.07.013

Enhancing the interaction between annexin-1 and formyl peptide receptors regulates microglial activation to protect neurons from ischemia-like injury

J Neuroimmunol. 2014 Nov 15;276(1-2):24-36. doi: 10.1016/j.jneuroim.2014.07.013. Epub 2014 Jul 30.

Authors

Zhen Zhao Luo¹, Yan Gao¹, Ning Sun¹, Yin Zhao¹, Jing Wang¹, Bo Tian¹, Jing Shi²

Affiliations

¹ Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China; Key Laboratory of Neurological Diseases of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
² Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China; Key Laboratory of Neurological Diseases of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China. Electronic address: sj@mails.tjmu.edu.cn.

PMID: 25115219
DOI: 10.1016/j.jneuroim.2014.07.013

Abstract

As the immune cells of the brain, microglia are crucial for the maintenance of brain function. The aims of the present study were to determine whether and how annexin-1 is able to affect microglial phenotype and migration in the lesion microenvironment. In the current experiment, we enhanced the interaction between annexin-1 and formyl peptide receptors in microglia and analyzed the function. We found that annexin-1 could polarize microglia to a beneficial phenotype and promote microglial migration to protect neurons from ischemia-like injury, and the annexin-1-mediated neuroprotective effect was dependent on the release of glutamate and ATP from the injured neurons.

Keywords: Annexin-1; Microglia; Migration; Neuronal injury; Phenotype.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / pharmacology
Animals
Annexin A1 / genetics
Annexin A1 / metabolism*
Annexin A1 / pharmacology
Calcium / metabolism
Cell Movement / physiology
Cell Survival / drug effects
Cells, Cultured
Cerebral Cortex / cytology
Dose-Response Relationship, Drug
Embryo, Mammalian
Glucose / deficiency
Glutamic Acid / pharmacology
Hypoxia / prevention & control
Mice
Mice, Inbred BALB C
Microglia / drug effects
Microglia / metabolism*
Neurons / drug effects
Neurons / metabolism*
Peptides / pharmacology
Rats
Rats, Sprague-Dawley
Receptors, Formyl Peptide / genetics
Receptors, Formyl Peptide / metabolism*
Time Factors
Up-Regulation / drug effects
Up-Regulation / genetics
Up-Regulation / physiology*

Substances

Annexin A1
Peptides
Receptors, Formyl Peptide
annexin A1 peptide (2-26)
Glutamic Acid
Adenosine Triphosphate
Glucose
Calcium