The exosome complex establishes a barricade to erythroid maturation

Blood. 2014 Oct 2;124(14):2285-97. doi: 10.1182/blood-2014-04-571083. Epub 2014 Aug 12.


Complex genetic networks control hematopoietic stem cell differentiation into progenitors that give rise to billions of erythrocytes daily. Previously, we described a role for the master regulator of erythropoiesis, GATA-1, in inducing genes encoding components of the autophagy machinery. In this context, the Forkhead transcription factor, Foxo3, amplified GATA-1-mediated transcriptional activation. To determine the scope of the GATA-1/Foxo3 cooperativity, and to develop functional insights, we analyzed the GATA-1/Foxo3-dependent transcriptome in erythroid cells. GATA-1/Foxo3 repressed expression of Exosc8, a pivotal component of the exosome complex, which mediates RNA surveillance and epigenetic regulation. Strikingly, downregulating Exosc8, or additional exosome complex components, in primary erythroid precursor cells induced erythroid cell maturation. Our results demonstrate a new mode of controlling erythropoiesis in which multiple components of the exosome complex are endogenous suppressors of the erythroid developmental program.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autophagy
  • Cell Differentiation
  • Epigenesis, Genetic
  • Erythroblasts / cytology
  • Erythrocytes / cytology*
  • Erythroid Cells / metabolism
  • Erythropoiesis / genetics
  • Exosomes / physiology*
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors / metabolism*
  • GATA1 Transcription Factor / metabolism*
  • Gene Expression Regulation
  • Mice
  • RNA / metabolism
  • Transcriptional Activation


  • Forkhead Box Protein O3
  • Forkhead Transcription Factors
  • FoxO3 protein, mouse
  • GATA1 Transcription Factor
  • Gata1 protein, mouse
  • RNA

Associated data

  • PDB/2NN6