Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy

Sci Rep. 2014 Aug 13;4:6044. doi: 10.1038/srep06044.


Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Artificial Organs*
  • Biocompatible Materials
  • Bone Regeneration
  • Cell Adhesion Molecules / biosynthesis
  • Dental Cementum / metabolism
  • Dental Implants*
  • Dental Sac / cytology
  • Dental Sac / physiology
  • Durapatite / chemistry
  • Extracellular Matrix Proteins / biosynthesis
  • Gene Expression Regulation
  • Immunohistochemistry
  • Mice
  • Mice, Inbred C57BL
  • Orthodontics, Corrective / methods*
  • Osteocalcin / biosynthesis
  • Periodontal Ligament / physiology
  • Periodontal Ligament / surgery
  • Tissue Engineering / methods*
  • Tooth / surgery
  • Tooth / transplantation*


  • Biocompatible Materials
  • Cell Adhesion Molecules
  • Dental Implants
  • Extracellular Matrix Proteins
  • F-spondin protein, mouse
  • Postn protein, mouse
  • Osteocalcin
  • Durapatite