Extended shelf life of random donor platelets stored for 7 days in platelet additive solution at different temperatures

Biomed J. Jul-Aug 2014;37(4):211-7. doi: 10.4103/2319-4170.117896.

Abstract

Background: Platelets are routinely stored in plasma for 5 days at an average temperature of 22°C. In the present study, the shelf life of random donor platelets was extended by storing for 7 days with and without additive solution at temperatures of 22°C, 18°C, and 16°C.

Methods: Random donor platelets were stored in 100% plasma and 20%/80% platelet additive solution. The data were compared using paired "t"- test. The confidence limit was kept at 95%, hence a "p" < 0.05 was considered to be statistically significant.

Results: Out of total 150 samples, 148 samples were analyzed and 2 were discarded due to the bacterial contamination on day 7 at 22°C without platelet additive solution. A significant difference in platelet count, platelet factor 3 (PF 3), glucose, lactate dehydrogenase (LDH), and platelet aggregation was observed on day 7 (p < 0.001) at 16°C in without platelet additive solution. In platelet additive solution, the mean values of platelet count, platelet distribution width (PDW), LDH, and pH showed no significant difference on day 7 at 22°C, 18°C, and 16°C. Only significant differences were observed in the levels of mean platelet volume (MPV), PF 3, glucose, and platelet aggregation on day 7 (p < 0.001) at 16°C of the storage period.

Conclusion: Random donor platelets functions are better maintained in platelet additive solution as compared to plasma at a lower temperature of 18°C but not at 16°C, on the 7 th day.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Platelets / metabolism*
  • Blood Preservation*
  • Humans
  • Pharmaceutical Solutions*
  • Platelet Aggregation / physiology*
  • Platelet Count
  • Temperature*
  • Time Factors
  • Tissue Donors

Substances

  • Pharmaceutical Solutions