Molecular Characterization of Two Opecoelid Trematodes From Fishes in the Gulf of Mexico, With a Description of a New Species of Helicometra

Acta Parasitol. 2014 Sep;59(3):405-12. doi: 10.2478/s11686-014-0258-7. Epub 2014 Aug 15.

Abstract

The plagioporine opecoelids Helicometra fasciata (Rudolphi, 1819) Odhner, 1902, and Macvicaria crassigula (Linton, 1910) Bartoli, Bray, and Gibson, 1989 have been reported from fishes in expansive geographic regions, disjointed from their type localities. New material of M. crassigula was collected from near its type locality as well as specimens resembling Helicometra fasciata sensu lato from three triglids in the Gulf of Mexico. Comparisons of the ribosomal DNA (rDNA) sequences, comprising the partial 18S rDNA, internal transcribed spacer region (= ITS1, 5.8S, and ITS2), and partial 28S rDNA gene, from M. crassigula and Helicometra fasciata sensu lato in the Gulf of Mexico were made with sequences deposited in GenBank from those species from the Mediterranean Sea. Results reveal that M. crassigula sensu stricto from the Gulf of Mexico is distinct from the two cryptic species of M. crassigula sensu lato from the Mediterranean Sea and Helicometra fasciata sensu lato in this study differs from H. fasciata sequences from the Mediterranean Sea, thus Helicometra manteri sp. nov. is described.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal Spacer / genetics
  • Fish Diseases / parasitology*
  • Gulf of Mexico
  • Molecular Sequence Data
  • Perciformes / parasitology*
  • Phylogeny
  • Sequence Analysis, DNA / veterinary
  • Species Specificity
  • Trematoda / classification*
  • Trematoda / genetics
  • Trematoda / isolation & purification
  • Trematode Infections / parasitology
  • Trematode Infections / veterinary*

Substances

  • DNA, Ribosomal
  • DNA, Ribosomal Spacer

Associated data

  • GENBANK/KJ701237
  • GENBANK/KJ701238
  • GENBANK/KJ701239