Phytochrome-interacting transcription factors PIF4 and PIF5 induce leaf senescence in Arabidopsis

Nat Commun. 2014 Aug 14;5:4636. doi: 10.1038/ncomms5636.

Abstract

Plants initiate senescence to shed photosynthetically inefficient leaves. Light deprivation induces leaf senescence, which involves massive transcriptional reprogramming to dismantle cellular components and remobilize nutrients. In darkness, intermittent pulses of red light can inhibit senescence, likely via phytochromes. However, the precise molecular mechanisms transducing the signals from light perception to the inhibition of senescence remain elusive. Here, we show that in Arabidopsis, dark-induced senescence requires phytochrome-interacting transcription factors PIF4 and PIF5 (PIF4/PIF5). ELF3 and phytochrome B inhibit senescence by repressing PIF4/PIF5 at the transcriptional and post-translational levels, respectively. PIF4/PIF5 act in the signalling pathways of two senescence-promoting hormones, ethylene and abscisic acid, by directly activating expression of EIN3, ABI5 and EEL. In turn, PIF4, PIF5, EIN3, ABI5 and EEL directly activate the expression of the major senescence-promoting NAC transcription factor ORESARA1, thus forming multiple, coherent feed-forward loops. Our results reveal how classical light signalling connects to senescence in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / physiology*
  • Arabidopsis / physiology*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / physiology*
  • Basic Helix-Loop-Helix Transcription Factors / genetics
  • Basic Helix-Loop-Helix Transcription Factors / physiology*
  • Basic-Leucine Zipper Transcription Factors / physiology
  • DNA-Binding Proteins
  • Light
  • Mutation / genetics
  • Nuclear Proteins / physiology
  • Phytochrome / physiology*
  • Plant Leaves / physiology*
  • Signal Transduction / physiology
  • Transcription Factors / physiology

Substances

  • ABI5 protein, Arabidopsis
  • Arabidopsis Proteins
  • Basic Helix-Loop-Helix Transcription Factors
  • Basic-Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • EEL protein, Arabidopsis
  • EIN3 protein, Arabidopsis
  • Nuclear Proteins
  • PIF4 protein, Arabidopsis
  • PIF5 protein, Arabidopsis
  • Transcription Factors
  • Phytochrome

Associated data

  • GEO/GSE52646