Sulfitobacter geojensis sp. nov., Sulfitobacter noctilucae sp. nov., and Sulfitobacter noctilucicola sp. nov., isolated from coastal seawater

Int J Syst Evol Microbiol. 2014 Nov;64(Pt 11):3760-3767. doi: 10.1099/ijs.0.065961-0. Epub 2014 Aug 13.

Abstract

Four Gram-stain-negative, aerobic, rod-shaped bacterial strains, MM-124, MM-126, NB-68 and NB-77, were isolated from the coastal seawater or a region with a bloom of sea sparkle around Geoje island in Korea. The sequence similarity values of the 16S rRNA gene between the isolates and Sulfitobacter mediterraneus DSM 12244(T) ranged from 97.7 to 98.2%, and phylogenetic relationships suggested that they belong to a phylogenetic branch that includes the genera Sulfitobacter and Roseobacter. The isoprenoid quinone of all three novel strains was ubiquinone-10 and the major fatty acid was cis-vaccenic acid, as in other species of the genus Sulfitobacter. However, there were several differences in the morphological, physiological and biochemical characteristics among the four strains and the reference species of the genus Sulfitobacter. Moreover, the average nucleotide identity values between the three sequenced isolates and the reference strains were below 76.33, indicating that genomic variation exists between the isolates and reference strains. Chemotaxonomic characteristics together with phylogenetic affiliations and genomic distances illustrate that strains MM-124, NB-68 and NB-77 represent novel species of the genus Sulfitobacter, for which the names Sulfitobacter geojensis sp. nov. (type strain MM-124(T) =KCTC 32124(T) =JCM 18835(T)), Sulfitobacter noctilucae sp. nov. (type strain NB-68(T) =KCTC 32122(T) =JCM 18833(T)) and Sulfitobacter noctilucicola sp. nov. (type strain NB-77(T) =KCTC 32123(T) =JCM 18834(T)) are proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oleic Acids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Rhodobacteraceae / classification*
  • Rhodobacteraceae / genetics
  • Rhodobacteraceae / isolation & purification
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Ubiquinone / analogs & derivatives
  • Ubiquinone / chemistry

Substances

  • DNA, Bacterial
  • Oleic Acids
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • cis-vaccenic acid
  • coenzyme Q10

Associated data

  • GENBANK/KC428714
  • GENBANK/KC428716
  • GENBANK/KC428717