Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids

J Lei; L T McLane; J E Curtis; J S Temenoff

doi:10.1039/C3BM60271K

Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids

Biomater Sci. 2014 May 1;2(5):666-673. doi: 10.1039/C3BM60271K.

Authors

J Lei¹, L T McLane², J E Curtis³, J S Temenoff⁴

Affiliations

¹ Woodruff School of Mechanical Engineering. Georgia Institute of Technology, Atlanta, Georgia, USA.
² School of Physics. Georgia Institute of Technology, Atlanta, Georgia, USA.
³ School of Physics. Georgia Institute of Technology, Atlanta, Georgia, USA ; Wallace H. Coulter Department of Biomedical Engineering. Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
⁴ Wallace H. Coulter Department of Biomedical Engineering. Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA ; Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.

Abstract

Mesenchymal stem cells therapies have the potential to treat many pathologies, however, controlling cell fate after implantation remains challenging. We have used a multilayer technology to graft a range of 5 μg/mL - 5 mg/mL heparin onto the surface of MSC aggregates. Heparin coating does not affect cell viability (seen through LIVE/DEAD staining), cell anti-inflammatory properties (seen through co-culture with activated monocytes)and facilitates sequestration by coated cells of a growth factor (TGF-β1) that remains bioactive. This system can maximize therapeutic potential of MSC-based treatments because the cell surface-loaded protein could both signal to the cells to influence transplanted cell fate and be released into the surrounding environment to help repair injured tissue.

Abstract

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