Abstract
It has been long appreciated that, during meiosis, DNA replication is coordinated with the subsequent formation of the double-strand breaks (DSBs) that initiate recombination, but a mechanistic understanding of this process was elusive. We now show that, in yeast, the replisome-associated components Tof1 and Csm3 physically associate with the Dbf4-dependent Cdc7 kinase (DDK) and recruit it to the replisome, where it phosphorylates the DSB-promoting factor Mer2 in the wake of the replication fork, synchronizing replication with an early prerequisite for DSB formation. Recruiting regulatory kinases to replisomes may be a general mechanism to ensure spatial and temporal coordination of replication with other chromosomal processes.
Copyright © 2014 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Cell Cycle Proteins / metabolism*
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DNA Breaks, Double-Stranded*
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DNA Replication*
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DNA-Binding Proteins / metabolism
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Meiosis*
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Nuclear Proteins / metabolism
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Phosphorylation
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Protein Serine-Threonine Kinases / metabolism*
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Recombinases / metabolism
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / metabolism*
Substances
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CSM1 protein, S cerevisiae
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Cell Cycle Proteins
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DNA-Binding Proteins
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Dbf4 protein, S cerevisiae
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Nuclear Proteins
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REC107 protein, S cerevisiae
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REC114 protein, S cerevisiae
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Recombinases
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Saccharomyces cerevisiae Proteins
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TOF1 protein, S cerevisiae
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CDC7 protein, S cerevisiae
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Protein Serine-Threonine Kinases
Associated data
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GEO/GSE52970
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GEO/GSE52987