Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid shear stress

Biotechnol Lett. 2014 Dec;36(12):2559-69. doi: 10.1007/s10529-014-1625-z. Epub 2014 Aug 17.


Improper fluid shear stress (FSS) can cause serious damages to bone marrow mesenchymal stem cells (MSCs). Mechano-growth factor (MGF) E peptide pretreatment was proposed to protect MSCs against FSS damage in this study. MSCs were exposed to FSS for 30 min after they were pretreated with MGF E peptide for 24 h. Then, the effects of MGF E peptide on the viability, proliferation and cell apoptosis of MSCs were investigated. MGF E peptide pretreatment could recover the cellular metabolic activity of MSCs reduced by 72 dyne cm(-2) FSS and had a synergistic effect with FSS on the cellular metabolic viability of MSCs under 24 and 72 dyne cm(-2) FSS. These results suggested that MGF E peptide pretreatment could be an effective method for the protection of FSS damage in bone tissue engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Biomechanical Phenomena*
  • Bone Marrow / physiology*
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Insulin-Like Growth Factor I / metabolism*
  • Mesenchymal Stem Cells / physiology*


  • mechano-growth factor E, human
  • Insulin-Like Growth Factor I