Effect of mesenchymal stem cells on the vascularization of the artificial site for islet transplantation in rats

Transplant Proc. Jul-Aug 2014;46(6):1963-6. doi: 10.1016/j.transproceed.2014.05.074.

Abstract

An adequate vascularization of the artificially created cavity is crucial for subsequent transplantation of isolated pancreatic islets. In a reported study, dynamic contrast-enhanced magnetic resonance imaging was used to assess the effect of mesenchymal stem cells on neoangiogenesis within connective tissue surrounding an implantable biocompatible device. The signal increase detected after injection of magnetic resonance contrast agent in each target region was considered to be an effect of contrast agent, which was related to the blood supply. To minimize the influence of variability in contrast agent application, all outcomes measured in the implanted devices were normalized to the signal intensity of kidney tissue. When supported by mesenchymal stem cells, the mean signal increase intra-abdominally was 42%, 41%, and 64% and within subcutaneously implanted devices was 23%, 54%, and 52% of that measured in kidney.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Contrast Media
  • Gadolinium
  • Islets of Langerhans Transplantation / instrumentation*
  • Kidney
  • Magnetic Resonance Imaging
  • Male
  • Mesenchymal Stem Cell Transplantation*
  • Neovascularization, Physiologic / physiology*
  • Omentum
  • Organometallic Compounds
  • Rats
  • Tissue Scaffolds*

Substances

  • Contrast Media
  • Organometallic Compounds
  • Gadolinium
  • gadofosveset trisodium