Effects of dimethylaminoethanol and compound amino acid on D-galactose induced skin aging model of rat

ScientificWorldJournal. 2014:2014:507351. doi: 10.1155/2014/507351. Epub 2014 Jul 14.

Abstract

A lasting dream of human beings is to reverse or postpone aging. In this study, dimethylaminoethanol (DMAE) and compound amino acid (AA) in Mesotherapy were investigated for their potential antiaging effects on D-galactose induced aging skin. At 18 days after D-gal induction, each rat was treated with intradermal microinjection of saline, AA, 0.1% DMAE, 0.2% DMAE, 0.1% DMAE + AA, or 0.2% DMAE + AA, respectively. At 42 days after treatment, the skin wound was harvested and assayed. Measurement of epidermal and dermal thickness in 0.1% DMAE + AA and 0.2% DMAE + AA groups appeared significantly thicker than aging control rats. No differences were found in tissue water content among groups. Hydroxyproline in 0.1% DMAE + AA, 0.2% DMAE + AA, and sham control groups was much higher than all other groups. Collagen type I, type III, and MMP-1 expression was highly upregulated in both 0.1% DMAE + AA and 0.2% DMAE + AA groups compared with aging control. In contrast, TIMP-1 expression levels of various aging groups were significantly reduced when compared to sham control. Coinjection of DMAE and AA into target tissue has marked antiaging effects on D-galactose induced skin aging model of rat.

MeSH terms

  • Amino Acids / pharmacology*
  • Animals
  • Collagen / genetics
  • Collagen / metabolism
  • Deanol / pharmacology*
  • Galactose / pharmacology
  • Male
  • Matrix Metalloproteinase 1 / genetics
  • Matrix Metalloproteinase 1 / metabolism
  • Rats
  • Rats, Wistar
  • Skin / drug effects*
  • Skin / metabolism
  • Skin Aging / drug effects*
  • Tissue Inhibitor of Metalloproteinase-1 / genetics
  • Tissue Inhibitor of Metalloproteinase-1 / metabolism

Substances

  • Amino Acids
  • Tissue Inhibitor of Metalloproteinase-1
  • Deanol
  • Collagen
  • Matrix Metalloproteinase 1
  • Galactose