Abstract
Escin, a natural mixture of triterpenoid saponin isolated from the seed of the horse chestnut, is reported to have a potent antiulcer activity against ethanol-induced gastric mucosal lesions. This study investigated the possible mechanisms underlying the gastroprotective effect of escin against indomethacin-induced gastric ulcer in mice. Gastric ulceration was induced by a single intragastric administration of indomethacin (18 mg/kg). The mice underwent intragastric treatment with escin at doses of 0.45, 0.9 or 1.8 mg/kg. Gastric lesion was estimated morphometrically and histopathologically 6 h after the indomethacin administration. The antioxidative parameters in gastric mucosa were measured. Moreover, the activity of myeloperoxidase and the contents of TNF-α, P-selectin and VCAM-1 in gastric tissues were determined. The results showed that escin protected gastric tissues against indomethacin-induced gastropathy as demonstrated from a reduction in the ulcer index and an attenuation of histopathologic changes. Escin caused significant reductions of the contents of malondialdehyde, TNF-α, P-selectin, VCAM-1 and myeloperoxidase activity. The altered activities of superoxide dismutase, catalase and glutathione peroxidase in the stomach tissues were also ameliorated by escin treatment. The present study demonstrated that escin had a protective effect against indomethacin-induced gastric ulcer in mice, not only by virtue of its antioxidant potential, but also due to its anti-inflammatory effect.
Keywords:
Anti-inflammatory; antioxidant; escin; gastric ulcer; indomethacin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / administration & dosage
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Anti-Inflammatory Agents, Non-Steroidal / adverse effects
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Anti-Inflammatory Agents, Non-Steroidal / antagonists & inhibitors*
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Anti-Inflammatory Agents, Non-Steroidal / therapeutic use
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Anti-Ulcer Agents / administration & dosage
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Anti-Ulcer Agents / therapeutic use*
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Antioxidants / administration & dosage
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Antioxidants / therapeutic use
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Dose-Response Relationship, Drug
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Escin / administration & dosage
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Escin / therapeutic use*
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Gastric Mucosa / drug effects*
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Gastric Mucosa / immunology
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Gastric Mucosa / metabolism
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Gastric Mucosa / pathology
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H(+)-K(+)-Exchanging ATPase / chemistry
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H(+)-K(+)-Exchanging ATPase / metabolism
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Indomethacin / adverse effects
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Indomethacin / antagonists & inhibitors*
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Lipid Peroxidation / drug effects
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Mice
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Oxidoreductases / antagonists & inhibitors
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Oxidoreductases / metabolism
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P-Selectin / antagonists & inhibitors
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P-Selectin / chemistry
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P-Selectin / metabolism
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Protective Agents / administration & dosage
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Protective Agents / therapeutic use*
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Random Allocation
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Severity of Illness Index
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Stomach / drug effects
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Stomach / immunology
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Stomach / pathology
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Stomach Ulcer / chemically induced
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Stomach Ulcer / metabolism
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Stomach Ulcer / pathology
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Stomach Ulcer / prevention & control*
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Tumor Necrosis Factor-alpha / agonists
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Tumor Necrosis Factor-alpha / antagonists & inhibitors
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Tumor Necrosis Factor-alpha / metabolism
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Vascular Cell Adhesion Molecule-1 / chemistry
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Vascular Cell Adhesion Molecule-1 / metabolism
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Anti-Ulcer Agents
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Antioxidants
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P-Selectin
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Protective Agents
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Tumor Necrosis Factor-alpha
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Vascular Cell Adhesion Molecule-1
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Escin
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Oxidoreductases
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H(+)-K(+)-Exchanging ATPase
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Indomethacin