Efficient quantification and characterization of bacterial outer membrane derived nano-particles with flow cytometric analysis

Int J Med Microbiol. 2014 Nov;304(8):1032-7. doi: 10.1016/j.ijmm.2014.07.012. Epub 2014 Jul 27.


There currently exists no efficient and easy method for size profiling and counting of membranous nano-scale particles, such as bacterial outer membrane vesicles (OMVs). We present here a cost-effective and fast method capable of profiling and counting small sample volumes of nano-scale membranous vesicles with standard laboratory equipment without the need for any washing steps. OMV populations of different bacterial species are compared and even subpopulations of OMVs can be identified after a simple labelling procedure. Counting is possible over three orders of magnitude without any changes to the protocol. Protein contaminations do not alter the described measurements.

Keywords: Escherichia coli; Flow cytometry; OMV; OMV enumeration; OMV quantification; Outer membrane vesicles.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteria / chemistry*
  • Bacteriological Techniques / methods
  • Cell-Derived Microparticles / chemistry*
  • Flow Cytometry / methods*
  • Nanoparticles / analysis*