Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies

BMC Biotechnol. 2014 Aug 21:14:78. doi: 10.1186/1472-6750-14-78.

Abstract

Background: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip.

Results: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications.

Conclusions: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Microarray Analysis / economics
  • Microarray Analysis / instrumentation*
  • Microarray Analysis / methods*
  • Microbiota*
  • Polymerase Chain Reaction
  • Reproducibility of Results