Formation of nuclear bodies by the lncRNA Gomafu-associating proteins Celf3 and SF1

Genes Cells. 2014 Sep;19(9):704-21. doi: 10.1111/gtc.12169.


Gomafu/MIAT/Rncr2 is a long noncoding RNA that has been proposed to control retinal cell specification, stem cell differentiation and alternative splicing of schizophrenia-related genes. However, how Gomafu controls these biological processes at the molecular level has remained largely unknown. In this study, we identified the RNA-binding protein Celf3 as a novel Gomafu-associating protein. Knockdown of Celf3 led to the down-regulation of Gomafu, and cross-link RNA precipitation analysis confirmed specific binding between Celf3 and Gomafu. In the neuroblastoma cell line Neuro2A, Celf3 formed novel nuclear bodies (named CS bodies) that colocalized with SF1, another Gomafu-binding protein. Gomafu, however, was not enriched in the CS bodies; instead, it formed distinct nuclear bodies in separate regions in the nucleus. These observations suggest that Gomafu indirectly modulates the function of the splicing factors SF1 and Celf3 by sequestering these proteins into separate nuclear bodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CELF Proteins
  • Cell Line, Tumor
  • Cell Nucleus / metabolism*
  • Cell Nucleus / ultrastructure
  • DNA-Binding Proteins / metabolism*
  • Humans
  • RNA Splicing Factors
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Transcription Factors / metabolism*


  • CELF Proteins
  • CELF3 protein, human
  • DNA-Binding Proteins
  • Miat long non-coding RNA
  • RNA Splicing Factors
  • RNA, Long Noncoding
  • RNA-Binding Proteins
  • SF1 protein, human
  • Transcription Factors