Litoreibacter Ponti Sp. Nov., Isolated From Seawater

Int J Syst Evol Microbiol. 2014 Nov;64(Pt 11):3810-3815. doi: 10.1099/ijs.0.066654-0. Epub 2014 Aug 21.

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GJSW-31(T), was isolated from seawater from the South Sea, South Korea. The novel strain grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain GJSW-31(T) clustered with the type strains of species of the genus Litoreibacter. Strain GJSW-31(T) exhibited 16S rRNA gene sequence similarity values of 95.2-98.5% to the type strains of species of the genus Litoreibacter and sequence similarities of less than 96.18% to type strains of the other species with validly published names. Strain GJSW-31(T) contained Q-10 as the predominant ubiquinone and C(18:1)ω7c as the major fatty acid. The major polar lipids of strain GJSW-31(T) were phosphatidylcholine, phosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain GJSW-31(T) was 62.5 mol% and its DNA-DNA relatedness values with the type strains of Litoreibacter albidus, Litoreibacter janthinus, Litoreibacter meonggei and Litoreibacter ascidiaceicola were 13-23%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJSW-31(T) is separate from other species of the genus Litoreibacter. On the basis of the data presented, strain GJSW-31(T) is considered to represent a novel species of the genus Litoreibacter, for which the name Litoreibacter ponti sp. nov. is proposed. The type strain is GJSW-31(T) ( =KCTC 42114(T) =NBRC 110379(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Rhodobacteraceae / classification*
  • Rhodobacteraceae / genetics
  • Rhodobacteraceae / isolation & purification
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Ubiquinone / analogs & derivatives
  • Ubiquinone / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • coenzyme Q10

Associated data

  • GENBANK/KJ729031