Tissue-specific expression of three types of beta-protein precursor mRNA: enhancement of protease inhibitor-harboring types in Alzheimer's disease brain

Biochem Biophys Res Commun. 1989 Dec 29;165(3):1406-14. doi: 10.1016/0006-291x(89)92760-5.

Abstract

Expression of three types of mRNA encoding amyloid beta-protein precursor (APP) in various tissues was analysed, using a ribonuclease protection assay, with special reference to Alzheimer's disease (AD). The total content and the proportion of APP mRNAs were specific to each tissue. Among eight tissues examined, the brain was distinct in that the expression level was highest and APP695 mRNA was expressed in abundance. The ratio of APP770/APP751/APP695 mRNAs was approximately 1:10:20 in the cerebral cortex of control brain. The proportions of APP770 mRNA and APP770-plus-APP751 mRNAs increased up to 2.6- and 1.4-fold, respectively, in various regions of AD brain compared with control. The enhanced expression of protease inhibitor-harboring types (APP770 and APP751) may disturb the balance between biosynthesis and degradation of APPs and ultimately lead to accumulation of beta-protein as amyloid.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / metabolism*
  • Amyloid / genetics*
  • Amyloid beta-Protein Precursor
  • Brain Chemistry*
  • Cerebral Cortex / analysis
  • Gene Expression*
  • Humans
  • Male
  • Protease Inhibitors*
  • Protein Precursors / genetics*
  • RNA, Messenger / analysis*
  • Ribonucleases
  • Tissue Distribution

Substances

  • Amyloid
  • Amyloid beta-Protein Precursor
  • Protease Inhibitors
  • Protein Precursors
  • RNA, Messenger
  • Ribonucleases