Transient expression of the angiotensin II receptor: a rapid and functional analysis of a calcium-mobilizing seven-transmembrane domain receptor in COS-7 cells

Biochem Biophys Res Commun. 1989 Dec 29;165(3):935-41. doi: 10.1016/0006-291x(89)92693-4.


The mas oncogene/angiotensin II receptor was subcloned into a mammalian expression vector pCDM8 and used to transiently transfect monkey kidney derived COS-7 cells. As a result, the mas transfected COS-7 cells expressed a functional angiotensin II receptor capable of transducing an increase in intracellular Ca2+ following stimulation with angiotensin II. The angiotensin II stimulated changes in Ca2+ could be measured 24 hours after transfection in both a fluorimeter and a fluorescence activated cell sorter. These results describe a rapid method for the functional analysis of the 7-transmembrane domain receptor genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Angiotensin II / pharmacology*
  • Animals
  • Calcium / metabolism*
  • Cell Line
  • Cell Membrane / metabolism*
  • Cloning, Molecular
  • Epinephrine / pharmacology
  • Gene Expression*
  • Genetic Vectors
  • Haplorhini
  • Kidney
  • Receptors, Angiotensin / genetics*
  • Transfection


  • Receptors, Angiotensin
  • Angiotensin II
  • Calcium
  • Epinephrine