Abstract
The in vitro generation of human cytotoxic T lymphocytes (CTL) is a reliably approach useful not only to assess intrinsic CD8(+) T cell responses in individuals but also to screen immunogenic antigens that could be considered as candidates for adoptive immunotherapeutic approaches. In vitro methods to expand CTL require culturing naïve T cells with antigen-presenting cells (APC) as stimulator cells that express specific antigens. Here, we describe the protocol for generating CTL against target antigens presented by monocyte-derived dendritic cells (DCs).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Neoplasm / immunology
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CD3 Complex / immunology
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Cell Differentiation / immunology
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Cells, Cultured
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Coculture Techniques
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Dendritic Cells / cytology
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Dendritic Cells / immunology*
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
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HLA-A2 Antigen / immunology
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Humans
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Immunotherapy, Adoptive
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Interleukin-4 / pharmacology
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Lymphocyte Activation / immunology*
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Monocytes / cytology*
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T-Lymphocytes, Cytotoxic / cytology*
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T-Lymphocytes, Cytotoxic / immunology*
Substances
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Antigens, Neoplasm
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CD3 Complex
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HLA-A2 Antigen
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Interleukin-4
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Granulocyte-Macrophage Colony-Stimulating Factor