Performance of the QuantiFERON-TB gold interferon gamma release assay among HIV-infected children in Botswana

J Int Assoc Provid AIDS Care. 2015 Jan-Feb;14(1):4-7. doi: 10.1177/2325957414547432. Epub 2014 Aug 22.


Interferon gamma release assays (IGRAs) are poorly studied in HIV-infected children. The authors prospectively evaluated QuantiFERON-TB Gold results and family-described tuberculosis (TB) risk factors in 100 HIV-infected children in Botswana. Median age was 10.2 years; 58 were girls, 92 had received the Bacillus Calmette-Guérin (BCG) vaccine, 98 were receiving antiretroviral therapy, and the median body mass index was 15.8 kg/m(2). Eighty-nine children had undetectable viral loads and the median CD4 count was 962 cells/mm(3). Eighteen children had been treated for TB in the last 3 years. In the last 3 years, 36 (including 9 with TB) had contact with persons with TB (26 within/15 outside the home and 5 had >1 contact). In all, 96 children had negative IGRAs, 3 were indeterminate, and 1 was positive. The positive IGRA was reported in a child treated for TB prior to 3 years. Interferon γ release assay positivity was rare in this pediatric cohort living in an area with a high prevalence of TB.

Keywords: HIV; interferon gamma release assay; latent tuberculosis infection; prevention; tuberculosis; tuberculosis risk factors.

MeSH terms

  • Adolescent
  • Botswana / epidemiology
  • Child
  • Child, Preschool
  • Cohort Studies
  • Female
  • HIV Infections / complications*
  • HIV Infections / epidemiology
  • Humans
  • Infant
  • Interferon-gamma Release Tests / methods*
  • Latent Tuberculosis / complications*
  • Latent Tuberculosis / diagnosis*
  • Latent Tuberculosis / epidemiology
  • Male