Objective of the study is to find the association between p21 rs1059234 polymorphisms and endometrial cancer in Han women from Northeast China. Genotyping of rs1059234 in the p21 gene was performed by sequencing and PCR-restriction fragment length polymorphism (PCR-RFLP) of peripheral blood samples in 263 endometrial cancer patients (case group) and 315 healthy subjects (control group). C allele frequency of rs1059234 was significantly increased in patients of the case group when compared with that in the control group (P = 0.039). The CC genotype frequency of rs1059234 was also significantly increased in patients of case group (0.335, vs. 0.219, P = 0.045). CC genotype increased the risk of endometrial cancer (OR = 1.589, 95%CI: 1.010-2.502). However, after corrected with regression analysis, the CC genotype did not increase the risk of endometrial cancer (OR = 1.623, 95% CI: 0.720-3.659, P = 0.243). Our study demonstrates that in Han women from Northeast China, there is no association between rs1059234 polymorphism of p21 gene and risk of endometrial cancer.