Hox protein interactions: screening and network building

Methods Mol Biol. 2014:1196:319-48. doi: 10.1007/978-1-4939-1242-1_20.


Understanding the mode of action of Hox proteins requires the identification of molecular and cellular pathways they take part in. This includes to characterize the networks of protein-protein interactions involving Hox proteins. In this chapter we propose a strategy and methods to map Hox interaction networks, from yeast two-hybrid and high-throughput yeast two-hybrid interaction screening to bioinformatic analyses based on the software platform Cytoscape.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computational Biology / methods
  • Databases, Protein
  • High-Throughput Screening Assays
  • Homeodomain Proteins / metabolism*
  • Protein Binding
  • Protein Interaction Mapping / methods*
  • Protein Interaction Maps
  • Reproducibility of Results
  • Two-Hybrid System Techniques
  • Yeasts / metabolism


  • Homeodomain Proteins