Production of interferon-gamma during infection of mice with Plasmodium chabaudi chabaudi

Immunobiology. 1989 Oct;179(4-5):353-65. doi: 10.1016/S0171-2985(89)80041-5.


An ELISA assay, designed to detect interferon-gamma (IFN-gamma) in the picogram range, was used to study the presence of IFN-gamma in serum and its production by T cells taken from C57BL/6 mice infected with Plasmodium chabaudi chabaudi. IFN-gamma was detectable in mouse plasma for two to three days before the peak of parasitaemia. Similarly, IFN-gamma production by T cells could be detected in vitro. In limiting dilution cultures, the production of IFN-gamma by as few as 1,000 T cells was detectable using this assay. The limiting dilution analysis revealed that a substantial IFN-gamma response by specific T cells occurs very early in a primary infection with P. chabaudi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • In Vitro Techniques
  • Interferon-gamma / analysis
  • Interferon-gamma / biosynthesis*
  • Interferon-gamma / blood
  • Lymphocyte Activation
  • Malaria / blood
  • Malaria / immunology*
  • Mice
  • Mice, Inbred C57BL
  • T-Lymphocytes / immunology


  • Interferon-gamma