Expression of cGMP-dependent protein kinase type I in mature white adipocytes

Biochem Biophys Res Commun. 2014 Sep 12;452(1):151-6. doi: 10.1016/j.bbrc.2014.08.071. Epub 2014 Aug 22.


The presence of cGMP-dependent protein kinase I (cGKI) in murine adipocytes has been questioned, although cGKI was implicated in the thermogenic program of fat cells (FCs) and to exert anti-hypertrophic/-inflammatory effects in white adipose tissue. Herein, cGKI was detected in adipocytes from control mice, whereas FCs from global cGKI knockouts (cGKI(-/-)) and cGKIα rescue (αRM) mice remained cGKI-negative. cGKI mutants exhibit decreased adipocyte size, plasma leptin levels and reduced body-weights as compared to litter-matched controls. Low abundance of adiponectin in WAT and plasma of αRM animals together with previously confirmed high IL-6 levels indicate a low-grade inflammation. However, αRMs were protected from streptozotocin-induced hyperglycemia. Our results suggest that cGMP/cGKI affects both glucose and FC homeostasis in more complex mode than previously thought.

Keywords: Adiponectin; Browning; Interleukin-6; Natriuretic peptides; PKG; cGMP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / enzymology*
  • Animals
  • Cyclic GMP-Dependent Protein Kinase Type I / metabolism*
  • Mice
  • Mice, Knockout


  • Cyclic GMP-Dependent Protein Kinase Type I