Abstract
Type I phosphatidylinositol 4-phosphate 5 kinase γ (PIP5KIγ) constitutes a major pathway for the generation of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) that regulates a variety of neuronal functions at both presynaptic and postsynaptic compartments. In this study, we examined the expression and localization of PIP5KIγ in the adult mouse retina. RT-PCR analysis revealed that PIP5KIγ_v2 was predominantly expressed in the retina while PIP5KIγ_v3 was also expressed faintly. Immunostaining of the adult mouse retina revealed intense PIP5KIγ-immunoreactivity in the inner and outer plexiform layers in a punctate manner. In the photoreceptor ribbon synapse, PIP5KIγ was highly concentrated at the periactive zone. These findings suggest that PIP5KIγ, especially PIP5KIγ_i2, is localized at the periactive zone, a functionally suitable compartment for the endocytosis of synaptic vesicles in photoreceptor ribbon synapses.
Keywords:
Active zone; Endocytosis; Phosphoinositide; Photoreceptor; Retina; Ribbon synapse.
Copyright © 2014 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Oxidoreductases
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Animals
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Calbindins / metabolism
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Clathrin / metabolism
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Co-Repressor Proteins
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DNA-Binding Proteins / metabolism
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Disks Large Homolog 4 Protein
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Guanylate Kinases / metabolism
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Male
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Membrane Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Microscopy, Immunoelectron
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Nerve Tissue Proteins / metabolism
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Phosphoproteins / metabolism
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Photoreceptor Cells / metabolism*
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Photoreceptor Cells / ultrastructure
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Protein Kinase C / metabolism
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Retina / cytology
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Synapses / metabolism*
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Synapses / ultrastructure
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Talin / metabolism
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Vesicular Inhibitory Amino Acid Transport Proteins / metabolism
Substances
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Bsn protein, mouse
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Calbindins
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Clathrin
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Co-Repressor Proteins
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DNA-Binding Proteins
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Disks Large Homolog 4 Protein
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Dlg4 protein, mouse
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Membrane Proteins
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Nerve Tissue Proteins
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Phosphoproteins
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Talin
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Vesicular Inhibitory Amino Acid Transport Proteins
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Viaat protein, mouse
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Alcohol Oxidoreductases
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Ctbp2 protein, mouse
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Phosphotransferases (Alcohol Group Acceptor)
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1-phosphatidylinositol-4-phosphate 5-kinase
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Protein Kinase C
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Guanylate Kinases