Nanoscale label-free bioprobes to detect intracellular proteins in single living cells

Sci Rep. 2014 Aug 26;4:6179. doi: 10.1038/srep06179.


Fluorescent labeling techniques have been widely used in live cell studies; however, the labeling processes can be laborious and challenging for use in non-transfectable cells, and labels can interfere with protein functions. While label-free biosensors have been realized by nanofabrication, a method to track intracellular protein dynamics in real-time, in situ and in living cells has not been found. Here we present the first demonstration of label-free detection of intracellular p53 protein dynamics through a nanoscale surface plasmon-polariton fiber-tip-probe (FTP).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gold / chemistry
  • HeLa Cells
  • Humans
  • Nanotubes / chemistry*
  • Single-Cell Analysis / instrumentation*
  • Surface Plasmon Resonance / instrumentation*
  • Tumor Suppressor Protein p53 / metabolism


  • TP53 protein, human
  • Tumor Suppressor Protein p53
  • Gold