A novel molecule Me6TREN promotes angiogenesis via enhancing endothelial progenitor cell mobilization and recruitment

Sci Rep. 2014 Aug 28;4:6222. doi: 10.1038/srep06222.

Abstract

Critical limb ischaemia is the most severe clinical manifestation of peripheral arterial disease. The circulating endothelial progenitor cells (EPCs) play important roles in angiogenesis and ischemic tissue repair. The increase of circulating EPC numbers by using mobilization agents is critical for obtaining a better therapeutic outcome in patients with ischemic disease. Here, we firstly report a novel small molecule, Me6TREN (Me6), can efficiently mobilize EPCs into the blood circulation. Single injection of Me6 induced a long-lasting increase in circulating Flk-1(+) Sca-1(+) EPC numbers. In a mouse hind limb ischemia (HLI) model, local intramuscular transplantation of these Me6-mobilized cells accelerated the blood flow restoration in the ischemic muscles. More importantly, systemic administration of Me6 notably increased the capillary density, arteriole density and regenerative muscle weight in the ischemic tissue of HLI. Mechanistically, we found Me6 reduced stromal cell-derived factor-1α level in bone marrow by up-regulation of matrix metallopeptidase-9 expression, which allowed the dissemination of EPCs into peripheral blood. These data indicate that Me6 may represent a potentially useful therapy for ischemic disease via enhancing autologous EPC recruitment and promote angiogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inducing Agents / pharmacology*
  • Animals
  • Apoptosis
  • Cell Movement / drug effects*
  • Cell Proliferation
  • Cells, Cultured
  • Chemokine CXCL12 / blood
  • Endothelial Progenitor Cells / physiology*
  • Enzyme Induction
  • Ethylamines / pharmacology*
  • Hindlimb / blood supply
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Human Umbilical Vein Endothelial Cells / physiology
  • Ischemia / drug therapy
  • Ischemia / physiopathology
  • Male
  • Matrix Metalloproteinase 9 / genetics
  • Matrix Metalloproteinase 9 / metabolism
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Neovascularization, Physiologic / drug effects*
  • Regional Blood Flow / drug effects
  • Up-Regulation

Substances

  • Angiogenesis Inducing Agents
  • Chemokine CXCL12
  • Ethylamines
  • tris(2-(dimethylamino)ethyl)amine
  • Matrix Metalloproteinase 9
  • Mmp9 protein, mouse