Metastatic Ability and Differentiative Properties of a New Cell Line of Human Embryonal Rhabdomyosarcoma (CCA)

Anticancer Res. Nov-Dec 1989;9(6):1943-9.


A new cell line (CCA) was established from a human embryonal rhabdomyosarcoma. It showed an "early" myogenic differentiation pattern: vimentin expression was found in 100% of cells, desmin in about 40% and myosin of the embryonic isoform in about 5%. Class I HLA expression on CCA cells was undeterctable but was greatly increased by in vitro treatment with human recombinant interferon-gamma and only marginally increased by human recombinant tumour necrosis factor-alfa. CCA cell line was tumorigenic in nude mice after either subcutaneous or intramuscular injection; macroscopic spontaneous metastases were not detected. The ability to induce metastatic nodules in the lungs was found when CCA cells were injected intravenously in cyclophosphamide-pretreated nude mice and, at low frequency, in untreated nude mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Line
  • Child
  • Culture Techniques / methods
  • Cyclophosphamide / therapeutic use
  • Humans
  • Interferon-gamma / pharmacology
  • Male
  • Mice
  • Mice, Nude
  • Neoplasm Metastasis
  • Neoplasm Transplantation
  • Recombinant Proteins
  • Rhabdomyosarcoma / drug therapy
  • Rhabdomyosarcoma / pathology*
  • Rhabdomyosarcoma / physiopathology
  • Teratoma / drug therapy
  • Teratoma / pathology*
  • Teratoma / physiopathology
  • Transplantation, Heterologous
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / drug effects


  • Recombinant Proteins
  • Interferon-gamma
  • Cyclophosphamide