Comparison of injection/dissection and injection/corrosion methods: example of vertebral veins in the transverse canal

Surg Radiol Anat. 2015 Apr;37(3):273-9. doi: 10.1007/s00276-014-1365-8. Epub 2014 Aug 29.

Abstract

Purpose: In order to contribute some new elements to the discussion on the organization of the vertebral veins inside the transverse canal, we compared two dissection techniques: injection/dissection and injection/corrosion. The aim of this study was to compare these two techniques to study the vertebral veins, and also to emphasize the importance of preserving specimens in anatomical museums.

Methods: Using the injection/dissection technique, latex was injected into ten specimens, then the transverse canal was opened to expose the vertebral veins and their anastomoses. Using the injection/corrosion technique, altufix was injected into eight specimens that were afterwards plunged in sulfuric acid and washed daily until complete corrosion was obtained.

Results: Both techniques showed concordant results. The vertebral veins were plexuous, located ventro-laterally to the artery and received constant metamerical branches at each level. The injection/dissection technique conserved the anatomical relationships allowing metamerical analysis of the vertebral veins. The injection/corrosion technique, however, failed to conserve these relationships but provided a precise visualization of smaller anastomosis.

Conclusions: By analyzing different aspects of the vertebral veins anatomy in the transverse canal, the two techniques complete each other. This study emphasizes the importance of preserving the anatomical preparations: in addition to the historical and cultural value of these preparations, they contain precious information that furthers our anatomical knowledge.

Publication types

  • Comparative Study

MeSH terms

  • Cadaver
  • Cervical Vertebrae / anatomy & histology
  • Cervical Vertebrae / blood supply*
  • Coloring Agents / analysis
  • Corrosion
  • Dissection / methods*
  • Female
  • Humans
  • Injections / methods
  • Male
  • Museums
  • Tissue Preservation / methods*
  • Veins / anatomy & histology*
  • Vertebral Artery / anatomy & histology

Substances

  • Coloring Agents