Pedobacter Pituitosus Sp. Nov., Isolated From a Waterfall

Int J Syst Evol Microbiol. 2014 Nov;64(Pt 11):3838-3843. doi: 10.1099/ijs.0.065235-0. Epub 2014 Aug 28.

Abstract

A taxonomic study was carried out on a novel bacterial strain, designated MIC2002(T), which was isolated from Wibong falls in Korea. Cells were Gram-stain-negative, aerobic, non-motile and rods, 0.3-0.5 µm wide and 4.0-5.0 µm long. The optimum temperature and pH range for growth were 25 °C and pH 6.5-7.0, respectively. Catalase and oxidase activities were positive. Flexirubin pigments were not produced. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the genus Pedobacter, with Pedobacter daechungensis as its closest relative, with a similarity of 94.4%. It contained iso-C(15:0), anteiso-C(15:0), C(16:0), summed feature 3 (C(16:1)ω6c and/or C(16:1)ω7c) and iso-C(17:0) 3-OH as the major fatty acids and menaquinone MK-7 as isoprenoid quinone. The polar lipid profile of strain MIC2002(T) revealed the presence of phosphatidylethanolamine and an unknown lipid. The DNA G+C content of the strain was 34.7 mol%. On the basis of the evidences presented, it was concluded that strain MIC2002(T) represents a novel species of the genus Pedobacter within the family Sphingobacteriaceae, for which the name Pedobacter pituitosus sp. nov. is proposed. The type strain is MIC2002(T) ( =KACC 17064(T) =JCM 18729(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Fresh Water / microbiology
  • Molecular Sequence Data
  • Pedobacter / classification*
  • Pedobacter / genetics
  • Pedobacter / isolation & purification
  • Phosphatidylethanolamines / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry
  • Water Microbiology*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phosphatidylethanolamines
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • phosphatidylethanolamine
  • vitamin MK 7

Associated data

  • GENBANK/JX978785