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. 2014 Aug;85(8):086104.
doi: 10.1063/1.4892379.

Note: Automated Optical Focusing on Encapsulated Devices for Scanning Light Stimulation Systems

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Note: Automated Optical Focusing on Encapsulated Devices for Scanning Light Stimulation Systems

L A Bitzer et al. Rev Sci Instrum. .

Abstract

Recently, a scanning light stimulation system with an automated, adaptive focus correction during the measurement was introduced. Here, its application on encapsulated devices is discussed. This includes the changes an encapsulating optical medium introduces to the focusing process as well as to the subsequent light stimulation measurement. Further, the focusing method is modified to compensate for the influence of refraction and to maintain a minimum beam diameter on the sample surface.

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