Adipogenic fate commitment of muscle-derived progenitor cells: isolation, culture, and differentiation

Methods Mol Biol. 2014;1213:229-43. doi: 10.1007/978-1-4939-1453-1_19.

Abstract

Skeletal muscle harbors several types of cells, among which a population of progenitors committed to the adipogenic lineage has only recently been identified. Potential sources of white and brown adipocytes, the latter representing a potential target to treat obesity, are of considerable interest to the field. Fluorescence-activated cell sorting (FACS) provides an elegant strategy for prospective isolation of closely defined cell populations. Here we describe a flow cytometric method to isolate muscle-resident adipogenic progenitor cells with a default potential to undergo white adipogenesis. We further describe an approach to induce commitment to a lineage of brown-like adipocytes upon exposure to bone morphogenetic protein 7 (BMP7).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology*
  • Adipogenesis*
  • Animals
  • Cell Culture Techniques
  • Cell Differentiation*
  • Cell- and Tissue-Based Therapy
  • Mice
  • Models, Animal
  • Muscle, Skeletal / cytology*
  • Stem Cells / cytology*