Characterization of the polycystic kidney disease 2 gene promoter

Genomics. 2014 Dec;104(6 Pt B):512-9. doi: 10.1016/j.ygeno.2014.08.006. Epub 2014 Aug 27.

Abstract

The key regulatory elements for PKD2 transcription remain unclear. To identify these core elements, we characterized porcine PKD2 promoter with bioinformatics and molecular tools and found porcine PKD2 promoter bearing typical features of enriched CpG and less TATA. Further studies demonstrated that the core region was located in fragment -483 to +100. Subsequent biophysical binding assays and mutation experiments revealed that G4 motif and Sp1 are critical regulators for mediating the transcription of porcine PKD2. Moreover, the same regulatory pattern was reproduced in human PKD2 promoter region, indicating the critical role of G4 and Sp1 in regulating PKD2.

Keywords: G-quadruplex; G4R1; PKD2; Sp1; Transcription.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • CpG Islands
  • HEK293 Cells
  • Humans
  • Molecular Sequence Data
  • Nucleotide Motifs
  • Promoter Regions, Genetic*
  • Protein Binding
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Serine-Threonine Kinases / metabolism
  • Pyruvate Dehydrogenase (Acetyl-Transferring) Kinase
  • Sp1 Transcription Factor / metabolism
  • Swine
  • Transcriptional Activation

Substances

  • Pyruvate Dehydrogenase (Acetyl-Transferring) Kinase
  • Sp1 Transcription Factor
  • Protein-Serine-Threonine Kinases