Cotranscriptional events in eukaryotic ribosome synthesis

Wiley Interdiscip Rev RNA. Jan-Feb 2015;6(1):129-39. doi: 10.1002/wrna.1263. Epub 2014 Aug 29.


Eukaryotic ribosomes are synthesized in a complex, multistep pathway. This begins with transcription of the rDNA genes by a specialized RNA polymerase, accompanied by the cotranscriptional binding of large numbers of ribosome synthesis factors, small nucleolar RNAs and ribosomal proteins. Cleavage of the nascent transcript releases the early pre-40S and pre-60S particles, which acquire export competence in the nucleoplasm prior to translocation through the nuclear pore complexes and final maturation to functional ribosomal subunits in the cytoplasm. This review will focus on the many and complex interactions occurring during pre-rRNA synthesis, particularly in budding yeast in which the pathway is best understood.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biological Transport
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • Macromolecular Substances / metabolism*
  • RNA Precursors / biosynthesis*
  • RNA, Ribosomal / biosynthesis*
  • Ribosomal Proteins / metabolism
  • Ribosomes / metabolism*
  • Transcription, Genetic*


  • Macromolecular Substances
  • RNA Precursors
  • RNA, Ribosomal
  • Ribosomal Proteins