¹³C-metabolic flux analysis for Escherichia coli

Yu Matsuoka; Kazuyuki Shimizu

doi:10.1007/978-1-4939-1170-7_16

¹³C-metabolic flux analysis for Escherichia coli

Methods Mol Biol. 2014:1191:261-89. doi: 10.1007/978-1-4939-1170-7_16.

Authors

Yu Matsuoka¹, Kazuyuki Shimizu

Affiliation

¹ Kyushu Institute of Technology, 680-4 Kawazu, Iizuka-shi, Fukuoka, 820-8502, Japan.

PMID: 25178796
DOI: 10.1007/978-1-4939-1170-7_16

Abstract

(13)C-Metabolic flux analysis ((13)C-MFA) is used here to study the effects of the knockout of such genes as pgi, zwf, gnd, ppc, pck, pyk, and lpdA on the metabolic changes in Escherichia coli cultivated under aerobic condition. The metabolic regulation mechanisms were clarified by integrating such information as fermentation data, gene expression, enzyme activities, and metabolite concentrations as well the result of (13)C-MFA.

MeSH terms

Aerobiosis
Carbon Isotopes* / metabolism
Escherichia coli / genetics
Escherichia coli / growth & development
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics
Fermentation
Gene Knockout Techniques
Glucose-6-Phosphate Isomerase / genetics
Metabolic Flux Analysis / methods*
Metabolic Networks and Pathways / genetics*
Metabolic Networks and Pathways / physiology
Models, Biological*

Substances

Carbon Isotopes
Escherichia coli Proteins
Glucose-6-Phosphate Isomerase
pgi protein, E coli