Quantitative analysis of single-molecule superresolution images

Curr Opin Struct Biol. 2014 Oct;28:112-21. doi: 10.1016/j.sbi.2014.08.008. Epub 2014 Aug 30.


This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10-50 nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Fluorescent Dyes
  • Microscopy / methods*
  • Microscopy / standards
  • Molecular Imaging / methods*
  • Molecular Imaging / standards


  • Fluorescent Dyes