Examining estrogen regulation of cancer stem cells through multicolor lineage tracing

Methods Mol Biol. 2014:1204:35-44. doi: 10.1007/978-1-4939-1346-6_4.

Abstract

We have designed a dual-color fluorescent reporter that can track microRNA expression in vitro, which can be used for lineage tracing experiments. We have used this system to track miR-140 promoter activity in breast cancer cells and to follow the impact of estrogen signaling in cancer stem cell subpopulations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Cloning, Molecular / methods
  • Estrogens / metabolism*
  • Female
  • Fluorescent Dyes / analysis
  • Fluorescent Dyes / metabolism
  • Gene Expression Regulation, Neoplastic
  • Genes, Reporter
  • Humans
  • MCF-7 Cells
  • MicroRNAs / genetics*
  • Microscopy, Fluorescence / methods
  • Neoplastic Stem Cells / metabolism*
  • Plasmids / genetics
  • Promoter Regions, Genetic*
  • Signal Transduction
  • Transfection / methods

Substances

  • Estrogens
  • Fluorescent Dyes
  • MicroRNAs
  • Mirn140 microRNA, human